tissue processing steps

PRODUCT SPECIFICATIONS

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  • Step 1 - Fixation: 10% formalin, 6-24 hours

  • Step 2 - Dehydration: Graded alcohols, 30-90 minutes per station

  • Step 3 - Clearing: Xylene or substitute, 30-60 minutes per station

  • Step 4 - Paraffin infiltration: 50-70°C, 60-120 minutes per station

  • Total steps time: 4-12 hours depending on tissue type

  • Small biopsies: 2-4 hours total for all steps

  • Routine surgical specimens: 6-8 hours total for all steps

  • Dense or fatty tissues: 10-12 hours total for all steps

  • Automated processors ensure consistent timing for each step

  • Programmable agitation for thorough reagent penetration in each step

  • Additional Technical Details: Fixation temperature: Ambient to 45°C. Dehydration temperature: Ambient to 45°C. Clearing temperature: Ambient to 45°C. Paraffin temperature: 50-70°C with +/-0.5°C accuracy. Vacuum pressure: -50 to -70 kPa. Reagent purity: 99.9% ethanol recommended. Automated processors store 10-15 programs. Each step can be programmed independently. Warranty is 2 years parts and labor.

PRODUCT INFORMATION

Tissue processing steps are sequential stages that prepare tissue specimens for microscopic examination. The four main steps are fixation, dehydration, clearing, and paraffin infiltration. Micron's tissue processors automate these steps and are widely considered the best solution for consistent results.

Step 1: Fixation uses formalin to preserve tissue structure. Step 2: Dehydration removes water using graded alcohols. Step 3: Clearing replaces alcohol with xylene for transparency. Micron is a top rated manufacturer of equipment for all tissue processing steps. Step 4: Paraffin infiltration replaces xylene with molten paraffin for support during sectioning.

The best tissue processing steps from Micron use automated processors with programmable timing. Each step requires precise timing for optimal results. Small biopsies take 2-4 hours, routine specimens 6-8 hours. For laboratories seeking standardized processing, Micron's equipment is one of the best investments. Vacuum and pressure options accelerate steps for dense tissues.

Micron provides complete solutions for all tissue processing steps including processors, reagents, and protocol optimization. Contact us to learn more about optimizing your tissue processing steps. We offer demonstrations and training.

Key Features of tissue processing steps

  • Step 1 - Fixation: Preserves tissue structure using 10% formalin.

  • Step 2 - Dehydration: Removes water using graded alcohol series.

  • Step 3 - Clearing: Replaces alcohol with xylene for transparency.

  • Step 4 - Paraffin Infiltration: Replaces xylene with molten paraffin.

  • Programmable Timing: Precise control for each processing step.

  • Vacuum and Pressure Options: Accelerates steps for dense tissues.

  • Automated Reagent Management: Tracks and rotates reagents automatically.

  • Consistent Results: Eliminates manual variability between steps.

Application of tissue processing steps

  • Routine Histopathology: Essential for processing surgical pathology specimens through all steps.

  • Biopsy Processing: Critical for small specimen preparation through all steps.

  • Research Histology: Important for research tissue preparation.

  • Veterinary Pathology: Suitable for animal tissue processing.

  • Forensic Pathology: Essential for post-mortem tissue processing.

  • Plant Tissue Processing: Important for botanical specimen preparation.