Fixation: 10% formalin, 6-24 hours depending on tissue size
Dehydration: Graded alcohols (70%, 80%, 95%, 100%), 30-90 minutes per station
Clearing: Xylene or substitute, 30-60 minutes per station
Paraffin infiltration: 50-70°C, 60-120 minutes per station
Total processing time: 4-12 hours depending on tissue type
Small biopsies: 2-4 hours total processing time
Routine surgical specimens: 6-8 hours total processing time
Dense or fatty tissues: 10-12 hours total processing time
Automated processors ensure consistent timing for each step
Programmable agitation for thorough reagent penetration
Additional Technical Details: Fixation temperature: Ambient to 45°C. Dehydration temperature: Ambient to 45°C. Clearing temperature: Ambient to 45°C. Paraffin temperature: 50-70°C with +/-0.5°C accuracy. Vacuum pressure: -50 to -70 kPa for accelerated processing. Reagent purity: 99.9% ethanol recommended. Reagent change frequency: Every 200-500 cassettes. Automated processors store 10-15 programs. Warranty is 2 years parts and labor.
Tissue processing procedure is a critical series of steps in histopathology that prepares tissue specimens for microscopic examination. The standard procedure includes fixation, dehydration, clearing, and paraffin infiltration. Micron's tissue processors automate this procedure and are widely considered the best solution for consistent results.
The procedure begins with fixation using 10% formalin to preserve tissue structure. Dehydration removes water using graded alcohols (70%, 80%, 95%, 100%). Clearing replaces alcohol with xylene for transparency. Micron is a top rated manufacturer of equipment for complete tissue processing procedures. The final step, paraffin infiltration, replaces xylene with molten paraffin for support during sectioning.
The best tissue processing procedure from Micron uses automated processors with programmable timing. Each step requires precise timing for optimal results. Small biopsies take 2-4 hours, while routine specimens take 6-8 hours. For laboratories seeking standardized procedures, Micron's automated processors are one of the best investments. Vacuum and pressure options accelerate processing for dense tissues.
Micron provides complete tissue processing procedure solutions including processors, reagents, and protocol optimization support. Contact us to learn more about optimizing your tissue processing procedure. We offer demonstrations and training.
Fixation Step: Preserves tissue structure using 10% formalin.
Dehydration Step: Removes water using graded alcohol series.
Clearing Step: Replaces alcohol with xylene for transparency.
Paraffin Infiltration Step: Replaces xylene with molten paraffin.
Programmable Timing: Precise control for each processing step.
Vacuum and Pressure Options: Accelerates processing for dense tissues.
Automated Reagent Management: Tracks and rotates reagents automatically.
Consistent Results: Eliminates manual variability in processing.
Routine Histopathology: Essential for processing surgical pathology specimens.
Biopsy Processing: Critical for small specimen preparation.
Research Histology: Important for research tissue preparation.
Veterinary Pathology: Suitable for animal tissue processing.
Forensic Pathology: Essential for post-mortem tissue processing.
Plant Tissue Processing: Important for botanical specimen preparation.
